Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Ginsenoside Rg3 Inhibits the Growth of Osteosarcoma and Attenuates Metastasis through the Wnt/ β -Catenin and EMT Signaling Pathway

doi: 10.1155/2020/6065124

Figure Lengend Snippet: Effect of Rg3 on the Wnt/ β -catenin signaling pathway. (a, b) The effects of Rg3 on the activity of different signaling pathways in 143B and MG63 cells were identified by luciferase reporter assay. (c, e) Western blot showing that Rg3 downregulated β -catenin, c-Myc, and Cyclin D1. (d, f) Relative protein expression of Wnt/ β -catenin signaling pathway in OS cells. Data are shown as mean ± SD ( n = 3). ∗ P < 0.05 vs. control, ∗∗ P < 0.01 vs. control, and ∗∗∗ P < 0.001 vs. control.

Article Snippet: The antibodies are following: rabbit anti-human MMP2, MMP7, and MMP9 (Affinity Biosciences, Zhenjiang, Jiangsu, China); rabbit anti-human E-cadherin and zinc finger E-box binding homeobox 1 (ZEB1) (ImmunoWay Biotechnology Company, Jiangsu, China); rabbit anti-human N-cadherin (Cell Signaling Technology, Danvers, MA, USA); rabbit anti-human Vimentin, Snail, Twist, β -catenin, c-Myc, and Cyclin D1 (Wanleibio, Shenyang, China); and mouse anti-human β -actin monoclonal antibody and secondary antibodies (Zhongshan Golden Bridge Biotechnology, Beijing, China).

Techniques: Activity Assay, Protein-Protein interactions, Luciferase, Reporter Assay, Western Blot, Expressing, Control